Akademik Veri Yönetim Sistemi
South African Journal of Botany, cilt.188, ss.207-218, 2026 (SCI-Expanded, Scopus)
This investigation evaluated the multifunctional therapeutic potential of Algerian Crataegus monogyna Jacq. ethanolic extract focusing on its antioxidant and urease-inhibitory properties. An ethanolic extract was otained through a green extraction process specifically ultrasound-assisted extraction, and subsequently characterized using a developed reverse-phase high performance liquid chromatography with photodiode array detection (RP-HPLC-PDA). The analysis identified eight key phenolic constituents, notably epicatechin, chlorogenic acid, caffeic acid, rutin, quercetin, gallic acid, chrysin, and pinocembrin. Quantification of total phenolic and flavonoid contents further highlighted the extract's chemical richness. Antioxidant efficacy was determined via seven complementary in vitro assays, including DPPH, FRAP, β-carotene bleaching, phenanthroline, DMSO alkaline, superoxide radical scavenging (pyrogallol), and hydroxyl radical inhibition, all of which confirmed the extract's substantial free radical neutralizing capacity. Additionally, the extract demonstrated marked urease inhibitory activity, with an IC₅₀ value of 9.54 ± 0.37 μg/mL. Molecular docking studies provided further support, showing strong binding interactions between rutin and the urease active site. These results align with the traditional medicinal uses of C. monogyna, particularly in cardiovascular and gastrointestinal therapies, and suggest that its bioactivity is largely due to its phenolic profile. Collectively, the combined antioxidant and urease inhibitory activities position Algerian C. monogyna as a promising natural candidate for the development of multi target therapies against oxidative stress and urease mediated disorders.