Investigation of protective effect of water extract of Turkish propolis against oxidative damage induced by tert-butyl hydroperoxide on the erythrocyte cells


Yiğit E. , Değer O. , Demir S. , Akbulut Çakıroğlu K. , Korkmaz K.

45th Apimondia Congress, İstanbul, Türkiye, 29 Eylül - 04 Ekim 2017, ss.527, İstanbul, Turkey, 29 September - 04 October 2017, pp.527

  • Publication Type: Conference Paper / Full Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.527

Abstract

AIM: The aim of this study is to evaluate the protective effect of water extract of Turkish propolis against tert-butyl hydroperoxide (t-BHP)-induced oxidative damage on the erythrocyte cells (RBCs). MATERIALS-METHODS: The erythrocyte cells were isolated from the blood obtained by the ethical committee approval from health individuals. The erythrocyte package obtained was divided into four groups as control, water extract of Turkish propolis, positive control (quercetin) and negative control (t-BHP) group. Red blood cells package as experimental model was treated firstly with propolis extract. Then t-BHP was added to the cells packages. Protective effects of water extract of Turkish propolis were invastigated by malondialdehyde (MDA, final product of lipid peroxidation), total oxidant capacity (TOC), total antioxidant capacity (TAC) and antioxidant enzyme activities of superoxide dismutase (SOD) and catalase (CAT). RESULTS: In the water extract of Turkish propolis group, MDA, CAT, SOD, TOC measurements were found to be significantly lower than those in the negative control group (p<0.05); whereas TAC measurements were found to be significantly higher than those in the negative control group (p<0.05). CONCLUSION: The protective effect of water extract of Turkish propolis against oxidative damage induced by t-BHP on erythrocytes was revealed. Decreased SOD and CAT activities may be explained by water extract of Turkish propolis extract have antioxidant compounds that they removes free radicals from the cells. In addition, findings obtained may lead to further in vivo and in vitro studies on cells.