45th Apimondia Congress, İstanbul, Türkiye, 29 Eylül - 04 Ekim 2017, ss.527, İstanbul, Turkey, 29 September - 04 October 2017, pp.527
AIM: The aim of this study is to evaluate the protective effect of water extract of Turkish propolis against tert-butyl hydroperoxide
(t-BHP)-induced oxidative damage on the erythrocyte cells (RBCs).
MATERIALS-METHODS: The erythrocyte cells were isolated from the blood obtained by the ethical committee approval from health individuals.
The erythrocyte package obtained was divided into four groups as control, water extract of Turkish propolis, positive control (quercetin) and
negative control (t-BHP) group. Red blood cells package as experimental model was treated firstly with propolis extract. Then t-BHP was added
to the cells packages. Protective effects of water extract of Turkish propolis were invastigated by malondialdehyde (MDA, final product of lipid
peroxidation), total oxidant capacity (TOC), total antioxidant capacity (TAC) and antioxidant enzyme activities of superoxide dismutase (SOD)
and catalase (CAT).
RESULTS: In the water extract of Turkish propolis group, MDA, CAT, SOD, TOC measurements were found to be significantly lower than those
in the negative control group (p<0.05); whereas TAC measurements were found to be significantly higher than those in the negative control
CONCLUSION: The protective effect of water extract of Turkish propolis against oxidative damage induced by t-BHP on erythrocytes was
revealed. Decreased SOD and CAT activities may be explained by water extract of Turkish propolis extract have antioxidant compounds that they
removes free radicals from the cells. In addition, findings obtained may lead to further in vivo and in vitro studies on cells.