Akademik Veri Yönetim Sistemi
European Biotechnology Congress 2023, Ljubljana, Slovenya, 4 - 06 Ekim 2023, ss.20-21
Feruloyl
esterases (FAEs), (EC 3.1.1.73) are a subclass of carboxylic acid esterases, a
class of hydrolases that catalyze the formation and breakdown of the ester bond
between the ferulate ester groups required for crosslinking between
hemicelluloses and hemicellulose-lignin. FAEs have gained great importance in
the textile, agriculture, food and pharmaceutical industries, paper and
cosmetics industries, and in many industries and medical fields such as
obtaining ferulic acid (FA) from industrial wastes.
In
this study, FAE of a thermophilic bacterium, Geobacillus thermoglucosidasius
DSM 2542T, expressed in Escherichia
coli (E.coli) by cloning into pET28a(+) expression
vector before, was used (GthFAE) for CLEA immobilization studies. The effects of
precipitating agents, crosslinking agent concentration, cross linking time,
effect of enzyme concentration, temperature and precipitation time were
observed for croslinking. After crosslinking, the stability of immobilized
enzyme in organic solvents were observed. The structure of the immobilized
enzyme was screened with SEM.
As a result of all optimization studies, it was determined that the
enzyme was precipitated in acetonitrile. In the presence of glutaraldehyde, the
activity increased as the cross-linking time and the enzyme concentration
increased. GthFAE was recovered aproximetaly 100% at the end of the immobilization.
Key words: Feruloyl esterase, Geobacillus,
immobilization