Akademik Veri Yönetim Sistemi
Turk Hijyen ve Deneysel Biyoloji Dergisi, cilt.72, sa.2, ss.115-122, 2015 (Scopus)
Objective: Blood stream infections (BSI) is one of the significant hospital-acquired infections that increase mortality and morbidity. Early diagnosis of BSI, identification of microrganisms that cause infection and analyzing antimicrobial sensitivity tests are important in terms of patient's prognosis. In this study microorganisms isolated from blood cultures and their antimicrobial sensitivity were investigated. Besides, to reveal the distribution of our hospital's BSI pathogens and to present their antimicrobial sensitivity paterns, were aimed. Methods: In this study blood culture samples collected at Kahramanmaraş Necip Fazi{dotless}l City Hospital between September 2012 - May 2014, were examined. Samples were incubated with BACTEC/90050 (Becton Dickinson, Maryland, the USA) automatization system. For the identification of microorganisms, Vitek version 2.0 (Biomerieux, France) automatization system was used in addition to conventional methods when necessary. Antibiotic sensitivity tests were studied in compliance with Clinical and Laboratory Standards Institute (CLSI) standards with Kirby-Bauer disc diffusion susceptibility test. Results: During our study period 2.923 blood cultures were analysed. Six hundred ninety seven (23,7%) samples gave reproductive signal as positive. We observed contamination in 89 (12.8%) of samples. In 2137 (73.1%) samples reproductive signal was not received. While repeating isolates were excluded from study, 584 (20.0%) isolates were included in the study. The most-common organisms causing BSIs were coagulase negative staphylococci (CNS) (58.2%), Escherichia coli (8%) and Acinetobacter spp. (7.9%). Methicillin resistance was detected in 54.9% of CNS isolates and in 34.4% Staphylococus aureus isolates. However vancomycin and linezolid resistance were not detected in both of the bacteria. For Enterococci, 55.6% penicilline resistance was determined. For one patient (4.5%) vancomycine resistance was detected, linezolid and teichoplamin resistance were not stated. Isolates belonging to Enterobacteriaceae family were sensitive to tigecycline. Among Klebsiella spp., 5.9% of the isolates were resistant to imipenem. 2.4% of Acinetobacter spp. isolates were resistant to tigecycline. Conclusion: To refer clinicians, there is need to make studies about distribution of microorganisms and their antibiotic sensitivity patterns which are isolated from blood cultures in certain time intervals for updating empirical treatment protocols and right usage of antibiotics.