Production of poly(3-hydroxyalkanoates) containing aromatic substituents by Pseudomonas oleovorans

Curley J., Hazer B., Lenz R., Fuller R.

MACROMOLECULES, vol.29, no.5, pp.1762-1766, 1996 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 29 Issue: 5
  • Publication Date: 1996
  • Doi Number: 10.1021/ma951185a
  • Journal Name: MACROMOLECULES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1762-1766
  • Karadeniz Technical University Affiliated: No


Pseudomonas oleovorans was grown separately on 5-(4'-tolyl)valeric acid, 5-(4'-ethylphenyl)valeric acid, 5-(4'-biphenyl)valeric acid, and 8-(4'-tolyl)octanoic acid either as the sole carbon source or as a coefeed with either nonanoic acid or 5-phenylvaleric acid. For polymer production, 5-(4'-tolyl)valeric acid was the most effective growth substrate of the five. It resulted in the production of poly-3-hydroxy-5-(4'-tolyl)valerate, a crystalline polymer with a glass transition temperature of 18 degrees C and a melting transition of 95 degrees C. This poly(3-hydroxyalkanoate) (PHA) is apparently the first example of a crystalline aromatic-containing bacterial PHA. When P. oleovorans was cofed an equimolar mixture of 5-phenylvaleric acid and 5-(4'-tolyl)valeric acid, the polymer produced contained 36 mol % of 3-hydroxy-5-phenylvalerate and 64 mol % of 3-hydroxy-5-(4'-tolyl)valerate, and it did not crystallize.