Objective: Measurement of central corneal thickness (CCT) plays an important role in the assessment of various ocular diseases. In this study we aimed to compare the measurements of the central corneal thickness by Stratus optical coherence tomography with ultrasound biomicroscopy in the healthy corneas. Material and Methods: A total of 54 eyes of 42 (19 females, 23 males) cases aged between 35 and 83 years underwent CCT measurements with Stratus OCT and UBM. All eyes were examined first with Stratus OCT and then by UBM. Pearson's correlation analysis and paired t-test were used to evaluate the relationship between the device results. Measurements were also compared by Bland-Altman analysis. Results: The mean CCT measured by Stratus OCT and UBM were 521.31 +/- 29.98 and 525.56 +/- 30.3 mu m, respectively. While the mean difference between two devices were 4.24 +/- 7.05 mu m, the measurements of two devices were significantly correlated (Pearson's coefficient = 0.973, p<0.0005). In Bland-Altman analysis, there was a high level of agreement between Stratus OCT and UBM. Conclusion: Corneal thickness measurements are influenced by the device of measurement. Generally, Stratus OCT underestimates corneal thickness compared to that measured with UBM. However the differences between two devices are not clinically significant. For this reason, measurement of the CCT can be performed by a standard retinal OCT device which is an accurate, noninvasive, and repeatable technique.