The random amplified polymorphic DNA (RAPD) technique offers a useful tool to detect DNA polymorphism. It can be used to distinguish different clones and cultivars. Thus, RAPD markers can detect enough polymorphism to differentiate among the wild cherry laurel (Prunus laurocerasus L.) and three cultivars. Here, we used this technique to determine the genetic similarity of cherry laurel and three cultivars grown in Turkey by using fourteen decamer primers. A total of 295 amplification products were formed, 22 of that were polymorphic. The genetic similarity among individuals was estimated based on Jaccard similarity index and cluster analysis. Jaccard similarity coefficient values ranged from 0.643 to 0.842. Two major clusters were obtained, one corresponding to the wild form and the second to the cultivars. The lowest similarity (0.643) obtained was found between the wild form and the cultivars, the highest similarity (0.842) obtained was between Prunus laurocerasus cv. "Oxygemmis" and P. laurocerasus cv. "Globigemmis". The similarity was 0.722 between the two cultivars and "Angustifolia".