Highly Efficient Spectrophotometric Determination of Cu2+ ion in Aqueous Medium Using a thiosemicarbazone–derivative Ligand


Başoğlu A., OCAK Ü., Iyidoğan A. K.

Journal of Fluorescence, vol.33, no.3, pp.1003-1015, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 3
  • Publication Date: 2023
  • Doi Number: 10.1007/s10895-022-03127-6
  • Journal Name: Journal of Fluorescence
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Biotechnology Research Abstracts, Chimica, Compendex, EMBASE, MEDLINE
  • Page Numbers: pp.1003-1015
  • Keywords: Aqueous medium, spectrophotometric determination, Cu2+, Thiosemicarbazone derivative, OPTICAL EMISSION-SPECTROMETRY, LIQUID-LIQUID-EXTRACTION, SOLID-PHASE EXTRACTION, COPPER, WATER, METALS, COMPLEXES, NICKEL, COBALT, CU(II)
  • Karadeniz Technical University Affiliated: Yes

Abstract

A new simple spectrophotometric method for the determination of Cu2+ ion was developed using an thiosemicarbazone compound, 2-{4-[Bis(2-chloroethyl)amino]benzylidene}-N-[(4-methylthio)phenyl]hydrazinecarbothioamide (TSC). A simultaneous color change was observed (from colorless to bright yellow) by the addition of Cu2+ ion to the TSC ligand solution. The maximum absorbance of the TSC ligand measured at 366 nm was decreased by the presence of Cu2+ ion. The graphs of absorbance obtained by means of the Job’s method and the molar-ratio method proposed a complex formation with a 1:2 Cu2+–TSC ligand stoichiometry. The molar-ratio method with emission measurements also confirmed the stoichiometry. The complex stability constant of TSC–Cu2+ complex (K) was evaluated to be 1.76 × 105. The proposed spectrophotometric method was associated with the change in absorbance at 366 nm owing to the interaction between the TSC ligand and Cu2+ ion. From the spectrophotometric titration data, it was pointed out that TSC ligand (1.5 × 10− 5 mol L-1) selectively reacted with Cu2+ ion in DMSO/water (1:1, v/v, citrate buffer at pH = 6.0). The calibration curve for Cu2+ ion was obtained with a good linearity in the range of 0.0191–0.3241 mg L-1. The detection limit for Cu2+ ion was 0.0063 mg L-1. The proposed method was achievemently implemented in real water samples (drink water, tap water and, distilled water). Satisfactory recoveries were confirmed at three different concentrations. The method presented a relative standard deviation (RSD%) of less than 3.08%.