Akademik Veri Yönetim Sistemi
V. International Enzyme and Bioprocess Days EBDays 2024 , İzmir, Türkiye, 27 - 29 Ağustos 2024, ss.29, (Özet Bildiri)
Pectinolytic enzymes include a group of enzymes that catalyze the degradation of pectin-containing
substrates. They are widely found in nature and are produced by bacteria, yeast, fungi and plants.
Pectinases are divided into two subgroups, hydrolases and lyases, according to their mechanism of
action. Pectins; are classified as pectic substances, protopectins and pectic acids. Pectin substances are
polysaccharides containing polygalacturonic acid, rhamnogalacturonic acid, galactans and
arabinogalactans in their structure.
In the screening of pectinase-producing bacteria, 15 different bacterial strains were screened on pectin
containing medium then fruit juice applications were conducted. The results indicated that LA1A
(Bacillus cereus), LA4A (Bacillus paramycoides), FAD2 (Pseudomonas stutzeri) exhibited high
pectinase production. Among these, the strain LA1A was found to be the most efficient pectinase
producer and was selected for further studies. Pectinase obtained from LA1A was tested on orange,
apricot, apple, peach and pineapple fruits, and total dissolved solids (TSS) were determined using a
digital refractometer, and dissolved solids were expressed as degrees Brix (°Brix). In addition to the
enzyme obtained from LA1A, a commercially available pectinase was applied to the fruits for
comparative purposes. Considering all the results, it was observed that although there were changes in
the rates of pectin degredation for each fruit, LA1A pectinase definitely have an pectinolytic activity
and increased the amount of dissolved solids in the fruit juices produced and can be suggested its
potential usage for industrial applications.