Micropropagation of three medicinal endemic species of Tripleurospermum (Asteraceae)
Plant Cell, Tissue and Organ Culture, cilt.166, sa.2, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 166 Sayı: 2
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11240-026-03560-6
- Dergi Adı: Plant Cell, Tissue and Organ Culture
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Compendex, Environment Index, Natural Science Collection (ProQuest), Biological Science Database (ProQuest)
- Anahtar Kelimeler: Cytogenetic stability, Micropropagation, T. heterolepis, T. repens, Tripleurospermum conoclinium
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
Efficient micropropagation protocols were developed for Tripleurospermum conoclinium, T. heterolepis, and T. repens, three medicinal, endemic species of Türkiye, to support their ex situ conservation and sustainable preservation. The effects of different cytokinin–auxin combinations (6-benzylaminopurine (BAP, 0.25, 0.5 and 1 mg L⁻¹), kinetin (KIN, 0.25, 0.5 and 1 mg L⁻¹), 6-(γ,γ-dimethylallylamino)-purine (2iP, 0.25, 0.5 and 1 mg L⁻¹) with indole-3-butyric acid (IBA, 0.1 mg L⁻¹)) and naphthaleneacetic acid (NAA, 0.1 mg L− 1) on shoot proliferation and elongation were evaluated on Murashige and Skoog (MS) medium. The highest shoot numbers were obtained on MS medium supplemented with 0.25 mg L⁻¹ BAP in combination with low NAA concentrations, reaching 3.20 shoots per explant in T. conoclinium and 5.25 shoots per explant in T. heterolepis, whereas T. repens produced a maximum of 3.80 shoots per explant under optimized PGR treatments. Shoot elongation was favored by kinetin or BAP combined with indole-3-butyric acid. Root induction was strongly auxin-dependent, with indole-3-butyric acid enhancing root number and secondary root formation, yielding up to 6.10 roots per shoot in T. conoclinium, 2.19 roots per shoot in T. heterolepis, and 4.95 roots per shoot in T. repens. Successfully rooted plantlets were acclimatized under ex vitro conditions, exhibiting high survival rates (82–95%) and normal flowering and achene production. Chromosome counting and flow cytometric analyses jointly confirmed the cytogenetic stability of in vitro regenerated plants, demonstrating stable chromosome numbers and conserved nuclear DNA contents in Tripleurospermum conoclinium (2n = 2x = 18), T. heterolepis (2n = 4x = 36), and T. repens (2n = 4x = 36) throughout culture and acclimatization.