This study is performed to evaluate the urinary iodine levels in pregnant women. Eighty pregnant women and 20 non-pregnant women were included into the study. In 29 pregnant women with goiter (group IA) and 51 pregnant women without goiter (group IB). Control group was divided into 2 subgroups: 10 non-pregnant women with goiter (group IIA) and 10 non-pregnant healthy women (group IIB). As a result, no significant difference in urinary iodine levels was found among groups IA, IB, IIA, IIB. The urinary iodine levels in pregnant women (group IB) in the 2nd and 3rd trimesters were significantly higher than those of the pregnant women in 1st trimester (respectively, p < 0.01, p < 0.05). In group IA, there was no significant difference in the urinary iodine levels between the trimesters. As a conclusion, although iodine deficiency is the main cause of endemic goiter in the Eastern Black Sea region, there was no increase in urinary iodine level in pregnant women with goiter. In addition to iodine, other possible agents for goiter formation in pregnant women should be investigated. Because urinary iodine levels in all groups were lower than the WHO's optimal level, the mass prophylaxis by iodine should be performed in pregnant as well as non-pregnant women.