Background and Purpose: Aim is to evaluate the antibacterial activity and physical properties of Biodentine containing chlorhexidine (CHX)/cetrimide (CT) mixtures at 24 h. Materials and Methods: Powder formulations of CHX (2.5%) and CT (2.5%) were added to the powder of Biodentine and served as experimental groups (EXP). Antibacterial free groups were designed as control (CNT). The antibacterial acitivity (AB) (n = 5; for each) of disc-shaped specimens against Streptococcus mutans and Lactobacillius casei and compressive strength (CS) (n = 10; for each) tests used both for groups after 24 h after polymerization. Statistical Analysis Used: Normality of data were tested with the ShapirouWilk test. Significant differences were analyzed with the MannuWhintey U test for both the methods (agar disk diffusion and compressive strength) at a significance level of P < 0.05. Results: Significantly higher antibacterial effects were obtained in EXP groups compared to the CNTs for both bacteria after 24 h, distinctly (P < 0.01). No differences were found between groups regarding the CS values (P 0.05). Conclusion: Since the incorporation of 5% CHX/CT to the Biodentine exhibited acceptable antibacterial activity without hampering compressive strength values at 24 h, this combination could be a promising alternative for eliminating the residual bacteria for indirect pulp-capping therapies.