Effects of quercetin on gentamicin-induced experimental testicular injury in rats

Biyik A. F., YULUĞ E., YENİLMEZ E., Kutlu A., ALVER A., ERDEM Ş.

Romanian Journal of Morphology and Embryology, vol.65, no.1, pp.69-80, 2024 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 65 Issue: 1
  • Publication Date: 2024
  • Doi Number: 10.47162/rjme.65.1.09
  • Journal Name: Romanian Journal of Morphology and Embryology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, MEDLINE
  • Page Numbers: pp.69-80
  • Keywords: apoptosis, gentamicin, oxidative stress, quercetin, testis
  • Karadeniz Technical University Affiliated: Yes


The purpose of this study was to investigate the effects of gentamicin (GEN) on the testis and whether quercetin (QUE) has any protective effect. Twenty-four adult male Sprague–Dawley rats were divided into equal four groups: control (0.9% saline solution), GEN (80 mg/kg GEN), QUE (50 mg/kg QUE) and GEN+QUE (80 mg/kg GEN + 50 mg/kg QUE). Histopathological (HP) evaluation of testis was performed, epididymal sperm parameters were analyzed and oxidative status was evaluated. The use of QUE improved the HP findings, such as decrease in the germinal epithelial thickness in the testicular tissue of the GEN group, decrease in the Johnsen’s tubular biopsy score (JTBS), increase in the rate of immature cell shedding tubules, and the apoptotic index (AI). In the GEN group, sperm count, and abnormal morphology increased compared to the control group; the viability and motility decreased according to the sperm analysis results. In the GEN+QUE group, QUE was found to improve sperm viability and morphology. In the GEN group, tissue malondialdehyde (MDA) levels increased while superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels decreased. Compared with the GEN+QUE group, it was found that the tissue MDA level decreased, while the levels of SOD, CAT and GPx increased. The results demonstrate that GEN impairs testicular structure and function, and QUE treatment can prevent this adverse effect.