Studies on pollen viability of Tripleurospermum ziganaense (Asteraceae) – a critically endangered endemic species from Türkiye

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Sağan R., İnceer H.

7th Symposium on EuroAsian Biodiversity (SEAB)–2024, Erzurum, Türkiye, 22 - 24 Ağustos 2024, ss.5-6, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Erzurum
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.5-6
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Pollen viability is one of the main factors affecting reproductive biology. Viable pollen grains are important for survival of the next plant generation and it play an important role for genetic diversity of the species with reproduction. Therefore, it can be helpful for conservation studies of threatened species. Tripleurospermum ziganaense Inceer and Hayırlıoglu-Ayaz (Asteraceae) is critically endangered (CR) endemic species in Türkiye due to several antropogenic pressures. In this study, we presented the results of pollen viability test of this species for the first time. In order to determine the rate of pollen viability orcein, tetrazolium (TTC) and safranine were used.  Plant materials were collected from Gümüşhane province where its natural population is located, during the vegetation period.  Pollen grains obtained from disk flowers at anthesis stage were stained with %1 solution of orcein, safranin and tetrazolium for 12 hours. Dark colored pollen grains were considered alive and others were considered non-living.  According to the results obtained, the viable pollen rate for T. ziganaense is 79±8.1% in safranin and 91±5.51% in TTC. However, it is not possible to distinguish between living and non-living with orcein.  In the study, it was determined that the pollen viability rate was over 70% in safranin and tetrazolium and these results indicate that T. ziganaense has high pollen viability. The present data obtained from pollen viability test can be used for reproductive biology and conservation acvities of the endangered endemic T. ziganaense as well as other treatened endemic species of Tripleurospermum.

 

Keywords: Tripleurospermum ziganaense, pollen viability, reproductive biology, conservation