Fatty acid composition of total leaf lipids of 23 species from five series of the genus Alchemilla L. was studied by GC. Fatty acids identified and quantified are C 12:0, C 14:0, C 16:0, C 18:0, C 20:0, C 22:0, C 24:0, C 26:0 and C 28:0 as saturated, and C 16:1, C 18:1, C 18:2 and C 18.3 as unsaturated with the major fatty acids C 18:2 and C 18:3. The highest content of fatty acid was quantified in A. hirtipedicellata (78.34%) for ser. Elatae, A. erytropoda (68.74%) for set. Pubescentes, A. rizensis (76.47%) for ser. Sericeae, A. dura (73.94%) for ser. Calycinae and A. pseudocartalinica (74.58%) for ser. Vulgares. The C 18:1 acid was determined in trace amounts while C 20:0, C 22:0, C 24:0 and C 28:0 acids were not determined in most of these species. Taxonomic value and phylogenetic implications of results are discussed.