Promoter analysis of the Chilo iridescent virus DNA polymerase and major capsid protein genes

Nalcacioglu R., Marks H., VLAK J. M., Demirbag Z., van Oers M.

VIROLOGY, vol.317, no.2, pp.321-329, 2003 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 317 Issue: 2
  • Publication Date: 2003
  • Doi Number: 10.1016/j.virol.2003.08.007
  • Journal Name: VIROLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.321-329
  • Keywords: Iridoviridae, iridovirus, Chilo iridescent virus, DNA polymerase, major capsid protein, helicase, promoter studies, luciferase, FROG VIRUS-3, MACROMOLECULAR-SYNTHESIS, RNA-POLYMERASE, GENOME, REPLICATION, SEQUENCE, DISEASE, TRANSACTIVATION, IDENTIFICATION, TRANSCRIPTION
  • Karadeniz Technical University Affiliated: Yes

Abstract

The DNA polymerase (DNApol) and major capsid protein (MCP) genes were used as models to study promoter activity in Chilo iridescent virus (CIV). Infection of Bombyx mori SPC-BM-36 cells in the presence of inhibitors of DNA or protein synthesis showed that DNApol, as well as helicase, is an immediate-early gene and confirmed that the major capsid protein (MCP) is a late gene. Transcription of DNApol initiated 35 nt upstream and that of MCP 14 nt upstream of the translational start site. In a luciferase reporter gene assay both promoters were active only when cells were infected with CIV. For DNApol sequences between position -27 and -6, relative to the transcriptional start site, were essential for promoter activity. Furthermore, mutation of a G within the sequence TTGTTTT located just upstream of the DNApol transcription initiation site reduced the promoter activity by 25%. Sequences crucial for MCP promoter activity are located between positions -53 and -29. (C) 2003 Elsevier Inc. All rights reserved.