Knock-Out Mutation of Cat2 Drastically Reduced The Melatonin-Mediated Resistance to Boron Toxicity in Arabidopsis thaliana


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Sağlam A., Sezgin Muslu A., Altuntaş C., Demiralay M., Kadioğlu A.

ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA, vol.65, no.2, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 65 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.24425/abcsb.2023.145595
  • Journal Name: ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts
  • Karadeniz Technical University Affiliated: Yes

Abstract

The role of catalase in resistance to boron toxicity after melatonin application (MEL) was investigated in Arabidopsis thaliana plants. Col-0 and cat2-2 plants were exposed to 50 µM MEL followed by boron toxicity (BT) in a medium containing 10 mM H3BO3. Pigment loss and accordingly chlorosis were reduced by melatonin under BT conditions, while they were more prominent in cat2-2 mutants. Moreover, TBARS and H2O2 contents, which increased due to BT, decreased as a result of melatonin application and the levels of these parameters in cat2-2 mutants were higher than the values in Col-0. Antioxidant enzyme activity of SOD and SOD1 gen transcript were induced by MEL under BT. Conversely, APX4, PER10 and CAT1 transcripts were down-regulated by MEL under BT. In addition, antioxidant enzyme activities and their transcript levels were lower than those of Col-0. Thus, we suggested that MEL scavenged ROS directly under BT. Melatonin also reduced the accumulation of boric acid in leaf tissues of Col-0, but not cat2-2. Finally, even though melatonin application provided a degree of endurance, the cat2 mutation resulted in increased sensitivity to BT.