KNOCK-OUT MUTATION OF<i> CAT2</i> DRASTICALLY REDUCED THE MELATONIN-MEDIATED RESISTANCE TO BORON TOXICITY IN <i>ARABIDOPSIS THALIANA</i>


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Sağlam A., Sezgin Muslu A., Altuntaş C., Demiralay M., Kadioğlu A.

ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA, vol.65, no.2, pp.7-19, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 65 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.24425/abcsb.2023.145595
  • Journal Name: ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts
  • Page Numbers: pp.7-19
  • Keywords: Arabidopsis, boron toxicity, catalase, chlorosis, melatonin
  • Karadeniz Technical University Affiliated: Yes

Abstract

The role of catalase in resistance to boron toxicity after melatonin application (MEL) was investigated in Arabidopsis thaliana plants. Col -0 and cat2-2 plants were exposed to 50 mu M MEL followed by boron toxicity (BT) in a medium containing 10 mM H3BO3. Pigment loss and accordingly chlorosis were reduced by melatonin under BT conditions, while they were more prominent in cat2-2 mutants. Moreover, TBARS and H2O2 contents, which increased due to BT, decreased as a result of melatonin application and the levels of these parameters in cat2-2 mutants were higher than the values in Col -0. Antioxidant enzyme activity of SOD and SOD1 gen transcript were induced by MEL under BT. Conversely, APX4, PER10 and CAT1 transcripts were down -regulated by MEL under BT. In addition, antioxidant enzyme activities and their transcript levels were lower than those of Col -0. Thus, we suggested that MEL scavenged ROS directly under BT. Melatonin also reduced the accumulation of boric acid in leaf tissues of Col -0, but not cat2-2. Finally, even though melatonin application provided a degree of endurance, the cat2 mutation resulted in increased sensitivity to BT.