The genus Brotherella Loeske ex Fleisch., B. erythrocaulis (Mitt.) Fleisch. (Sematophyllaceae) and Encalypta pilifera Funck in Sturm (Encalyptaceae) were reported for the first time from Turkey. These are also new records for the Mediterranean, the Middle East and Southwest Asia. The record of B. erythrocaulis is from Artvin region (Turkey), and that of E. pilifera is based on collections from Afyon, Burdur, Igdir and Izmir regions (Turkey). Descriptions, illustrations, geographic distribution, diagnostic characters of the species and comparisons with morphologically similar taxa are presented.