Chemical characterization of water and ethanolic extracts of Turkish propolis by HPLC-DAD and GC-MS


Bozkus T. N., DEĞER O., YAŞAR A.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.44, sa.1-2, ss.77-86, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 1-2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/10826076.2021.1883648
  • Dergi Adı: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.77-86
  • Anahtar Kelimeler: Ethanolic extract of propolis, GC-MS, HPLC-DAD, polyphenols, water extract of propolis
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

This study aims to determine the qualitative and quantitative contents of Turkish propolis collected from various regions of Turkey using high-performance liquid chromatography with the diode-array detector (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS) in water and ethanolic extracts. In HPLC-DAD analyses, it was determined that water extract of Turkish propolis contains phenolic acids such as caffeic acid (204.00 mu g/mL), trans-cinnamic, chlorogenic, and caffeoylquinic acids, responsible for its antioxidant activity; whereas, the ethanolic extract of Turkish propolis contains chrysin (641.33 mu g/mL), caffeic acid phenethyl ester (630.67 mu g/mL), pinocembrin (572.67 mu g/mL), galangin (534.11 mu g/mL), naringenin (372.39 mu g/mL), and also kaempferol, trans-cinnamic acid, caffeic acid, myricetin, and quercetin. GC-MS analyses showed that ethanolic extract of propolis contains caffeic acid by Rtx-1 column and the water extract of propolis contains quinic acid and ferulic acid by Rtx-5ms column. Various sugar derivatives were detected by both columns in water and ethanolic extracts of Turkish propolis. HPLC-DAD can be considered as a more effective method than GC-MS for the chemical characterization of propolis. Water extract of Turkish propolis can be a good source of raw materials for various sectors, as it is both cheap and has less health risk than ethanolic extract, and is suitable for human use.