Culturable Bacteria Isolation and Identification of Glyphodes pyloalis (Walker:1859) (Lepidoptera: Crambidae)
10th International Entomopathogens and Microbial Control Congress, Ankara, Türkiye, 27 - 29 Ekim 2026, ss.67, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Ankara
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.67
- Karadeniz Teknik Üniversitesi Adresli: Evet
Özet
Glyphodes pyloalis (Walker 1859) (Lepidoptera: Crambidae), commonly known as the lesser mulberry snout moth, is considered one of the most critical mulberry pests worldwide. First detected in Yalova province in 2018, we subsequently identified this pest in Trabzon in August 2024. Because mulberry (Morus sp.) can be cultivated across all regions of Türkiye, G. pyloalis poses a significant threat to future mulberry production. The objective of this study was to isolate and identify the bacteria associated with G. pyloalis. For this purpose, collected live and dead larvae were surface-sterilized, transferred into sterile Eppendorf tubes containing a phosphate-buffered solution, and thoroughly homogenized. Serial dilutions of the resulting homogenate were prepared, and aliquot samples were inoculated onto Nutrient Agar plates using the spread-plate technique. Following incubation, two distinct culturable bacterial isolates, designated as GP1 and GP2, were successfully isolated and purified based on their specific colony morphology. Gram staining revealed that isolate GP1 is a Gram-positive bacillus, whereas GP2 is a Gram-positive coccus. Biochemical characterization performed using API test kits preliminarily identified the isolates as Bacillus pumilus(GP1) and Staphylococcus xylosus(GP2). For molecular characterization, genomic DNA was extracted, and the 16S rRNA gene region was amplified via PCR and sequenced. Sequence alignment against the GenBank database confirmed the highest similarity to Bacillus pumilus for GP1 and Staphylococcus xylosusfor GP2. Future studies aim to evaluate the pathogenicity and virulence of these isolates against G. pyloalis to determine their potential as biological control agents
Keywords: Glyphodes pyloalis, culturable bacteria, mulberry, Bacillus