Synthesis of Ag Nanoparticles from Catechin Extract of Green Tea leaf

Serdar G., Gül Kılınç G., Sökmen M.

NATURAL SCIENCES USBİK 2021, Kayseri, Turkey, 26 - 28 February 2021

  • Publication Type: Conference Paper / Unpublished
  • City: Kayseri
  • Country: Turkey
  • Karadeniz Technical University Affiliated: Yes


The synthesis and characterization of Ag  nanoparticles using catechin extract of green tea leaves collected the Eastern Black Sea region (Of-TRABZON) were investigated in this study. Firstly, we isolated catechin extract using Supercritical Fluid Extraction. Subsequently, 10g of dried sample was shaken in 200 mL distilled water containing citric acid (0.1 M) for 90 min  at room temperature and then extracted in a laboratoary microwave device at 80 °C, 4 minutes,  600 W.  The extracts were completely dried in the lyophilizer and transferred to the SFE system. Caffeine was separated by applying at 250 bar, 60 ° C and for 3 hours and then the catechin extract was separated by using 0.5 mL / min ethanol, modified CO2-SFE extraction under the same conditions1. A different volume of catechin extract solution was added to AgNO3 solution (0,5- 2 mM) and the mixture was exposed to a household microwave at 90 W for 1–30 min. Plasmon resonance (SPR) absorption spectra were measured to determine optimum conditions (Shimadzu UVP-1240 spectrophotometer)2.