NATURAL SCIENCES USBİK 2021, Kayseri, Turkey, 26 - 28 February 2021, pp.31
The synthesis and
characterization of Ag nanoparticles
using catechin extract of green
tea leaves collected the Eastern Black Sea region
(Of-TRABZON) were investigated in this study. Firstly, we isolated catechin extract using Supercritical
Fluid Extraction. Subsequently, 10g
of dried sample was shaken in 200 mL
distilled water containing citric acid (0.1 M) for 90 min at
room temperature and then extracted in a laboratoary microwave device at 80 °C, 4 minutes, 600 W. The extracts were completely dried in the
lyophilizer and transferred to the SFE system. Caffeine was separated by
applying at 250 bar, 60 ° C and for 3 hours and then the catechin extract was
separated by using 0.5 mL / min ethanol, modified CO2-SFE extraction
under the same conditions1. A different volume of catechin extract
solution was added to AgNO3 solution (0,5- 2 mM) and the
mixture was exposed to a household microwave at 90 W for 1–30 min. Plasmon
resonance (SPR) absorption spectra were measured to determine optimum
conditions (Shimadzu UVP-1240 spectrophotometer)2.