The actions of ryanodine on Ca2+-activated conductances in rat cultured DRG neurones; evidence for Ca2+-induced Ca2+ release
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, vol.359, no.2, pp.81-91, 1999 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 359 Issue: 2
- Publication Date: 1999
- Doi Number: 10.1007/pl00005335
- Journal Name: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.81-91
- Karadeniz Technical University Affiliated: No
Abstract
The whole-cell recording technique was used to investigate the actions of a calcium release channel ligand, ryanodine, on calcium-activated chloride conductances, and to evaluate ryanodine-sensitive Ca2+-induced Ca2+ release from intracellular stores in cultured neonatal rat DRG neurones. The aim of the project was to use ryanodine as a pharmacological tool to evaluate calcium-induced calcium release in the cell bodies of cultured DRG neurones. Action potential after-depolarizations were attenuated by extracellular application of the chloride channel blocker, niflumic acid (10 mu M), and by ryanodine (10 mu M); these actions occurred without concurrent changes in evoked action potentials.