The actions of ryanodine on Ca2+-activated conductances in rat cultured DRG neurones; evidence for Ca2+-induced Ca2+ release


AYAR A. , Scott R.

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, vol.359, no.2, pp.81-91, 1999 (Journal Indexed in SCI) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 359 Issue: 2
  • Publication Date: 1999
  • Doi Number: 10.1007/pl00005335
  • Title of Journal : NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
  • Page Numbers: pp.81-91

Abstract

The whole-cell recording technique was used to investigate the actions of a calcium release channel ligand, ryanodine, on calcium-activated chloride conductances, and to evaluate ryanodine-sensitive Ca2+-induced Ca2+ release from intracellular stores in cultured neonatal rat DRG neurones. The aim of the project was to use ryanodine as a pharmacological tool to evaluate calcium-induced calcium release in the cell bodies of cultured DRG neurones. Action potential after-depolarizations were attenuated by extracellular application of the chloride channel blocker, niflumic acid (10 mu M), and by ryanodine (10 mu M); these actions occurred without concurrent changes in evoked action potentials.