The actions of ryanodine on Ca2+-activated conductances in rat cultured DRG neurones; evidence for Ca2+-induced Ca2+ release

AYAR A. , Scott R.

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, cilt.359, sa.2, ss.81-91, 1999 (SCI İndekslerine Giren Dergi) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 359 Konu: 2
  • Basım Tarihi: 1999
  • Doi Numarası: 10.1007/pl00005335
  • Sayfa Sayıları: ss.81-91


The whole-cell recording technique was used to investigate the actions of a calcium release channel ligand, ryanodine, on calcium-activated chloride conductances, and to evaluate ryanodine-sensitive Ca2+-induced Ca2+ release from intracellular stores in cultured neonatal rat DRG neurones. The aim of the project was to use ryanodine as a pharmacological tool to evaluate calcium-induced calcium release in the cell bodies of cultured DRG neurones. Action potential after-depolarizations were attenuated by extracellular application of the chloride channel blocker, niflumic acid (10 mu M), and by ryanodine (10 mu M); these actions occurred without concurrent changes in evoked action potentials.