Akademik Veri Yönetim Sistemi
XXXI MEETING OF BALKAN CLINICAL LABORATORY FEDERATION 35th NATIONAL BIOCHEMISTRY CONGRESS, Antalya, Türkiye, 28 Ekim - 01 Kasım 2024, cilt.49, ss.100
Objectives: The cafeteria (CAF) diet model, consists
of tasty but unhealthy food products that humans eat,
often used in animal models to mimic obesity in humans.
Denervation experiments in which nerve fibers
are cut used to study effects of the nervous system
on adipose tissue. Studies have shown that denervation
can correct metabolic disorders associated with
high-fat dietary intake. Our aim was to investigate the
effect of retroperitoneal adipose tissue denervation on
oxidant-antioxidant status in the liver in rats fed CAF
diet.
Methods: 24 male Wistar rats were used and 4 groups
were randomly assigned (n=6): control, control+denervation,
CAF diet, CAF diet+denervation. The first
two groups were fed with laboratory rodent chow and
other groups were fed CAF diet for 16 weeks. In the
denervation groups, retroperitoneal adipose tissue was
bilaterally denervated at week 8. At the end of period,
rats were sacrificed by decapitation. Superoxide dismutase
(SOD), catalase (CAT) and malondialdehyde
(MDA) were measured manually, while total antioxidant
status (TAS) and total oxidant status (TOS) were
measured using commercial kits.
Results: MDA level increased significantly in the
control+denervation group compared to the control
group (p<0.05). In CAF group, TOS and MDA levels
increased significantly compared to the control group,
while CAT level decreased significantly (p<0.05). In
the CAF+denervation group, TAS and SOD levels
increased significantly, while MDA level decreased
significantly (p<0.05) compared to the CAF group.
Conclusions: Denervation increased oxidative stress
in rats fed rodent chow, but decreased in rats fed CAF
diet in liver tissue.