Akademik Veri Yönetim Sistemi
The 9th International Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP-9), Ankara, Türkiye, 3 - 05 Mayıs 2023, ss.61
Numerous Ferula
species have been traditionally used for therapeutic purposes in alleviating
diabetic problems (1,2). In this study, in vivo antihyperglycemic effects of
root extracts prepared from Ferula
orientalis (FOE) and Ferula mervynii
(FME), an endemic species for Türkiye, and ferutinin (FT), the main compound of
Ferula species, were investigated. In vivo antihyperglycemic effect was
evaluated in male Balb/c mice by using
oral glucose tolerance test (OGTT) and streptozotocin (STZ)-induced type
1 diabetes model. In the OGTT method; FOE (200 mg/kg), FME (200 mg/kg), FT (100
mg/kg), glibenclamide (GLB, 5 mg/kg) or vehicle (10% DMSO) was administered
orally 30 min prior to glucose solution (2 g/kg) and blood glucose levels were
measured at different time intervals. To induce diabetes, STZ (50 mg/kg; i.p)
was administered to mice for five consecutive days and FOE (200 mg/kg), FME
(200 mg/kg), FT (100 mg/kg), GLB (5 mg/kg) or vehicle (10% DMSO) was orally
given for 14 days. Fasting blood glucose levels of the mice were measured on
the 8th and 15th day of the experiment. Serum insulin
level and caspase-3 expression in pancreatic tissue were detected by ELISA and
western blot, respectively. FOE, FME, FT and vehicle treatments did not exhibit
antihyperglycemic effects in OGTT and diabetic mice. However, GLB treatment
caused a significant decrease in blood glucose levels in both models
(p<0.05). A decrease in serum insulin levels and an increase in pancreatic
caspase-3 expression were detected in diabetic mice (p<0.05). Extracts and
FT treatments did not alter these parameters. In conclusion, we found that FOE,
FME and FT at tested doses did not possses in vivo antihyperglycemic effects.
Key Words: Diabetes,
Ferula mervynii, Ferula orientalis, ferutinin, in vivo, streptozotocin
Acknowledgements (not mandatory)
This study supported by the grants from the
Scientifc Research Project Coordination Unit of Karadeniz Technical
University (Project No. THD-2022-9970)
References
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