Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells

DEMİR, SELİM; TURAN, İBRAHİM; ALİYAZICIOĞLU, REZZAN; ÖZER YAMAN, SERAP; ALİYAZICIOĞLU, YÜKSEL

doi:10.1016/j.jpha.2018.05.003

Primula vulgaris extract induces cell cycle arrest and apoptosis in human cervix cancer cells

Atıf İçin Kopyala

DEMİR S., TURAN İ., ALİYAZICIOĞLU R., ÖZER YAMAN S., ALİYAZICIOĞLU Y.

JOURNAL OF PHARMACEUTICAL ANALYSIS, cilt.8, sa.5, ss.307-311, 2018 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 8 Sayı: 5
Basım Tarihi: 2018
Doi Numarası: 10.1016/j.jpha.2018.05.003
Dergi Adı: JOURNAL OF PHARMACEUTICAL ANALYSIS
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.307-311
Anahtar Kelimeler: Apoptosis, Cell cycle, Cervical cancer, Cytotoxicity, Primula vulgaris, NATURAL POLYPHENOLS, MEDICINAL-PLANTS, HUMAN LUNG, ANTIOXIDANT, FLAVONOIDS, LINES
Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Primula vulgaris belongs to the genus Primula, members of which are frequently used in folk medicine. Various studies have investigated the cytotoxic effect of different Primula species, but there have been limited studies on the cytotoxic effect of P. vulgaris. The aim of this study was to investigate the cytotoxic effects, and possible mechanisms involved, of P. vulgaris flower extract on human cervical cancer (HeLa) cells. The cytotoxic effect of the extract on HeLa cells was revealed using the MTT assay. Mechanisms involved in the extract's cytotoxic effect were then investigated in terms of apoptosis, mitochondrial membrane potential, and the cell cycle, using fluorometric methods. P. vulgaris flower extract exhibited selective cytotoxic effects against HeLa cells by arresting their cell cycle at the S phase, and inducing the number of apoptotic cells compared to normal fibroblast cells by reducing mitochondrial membrane potential in a concentration-dependent manner. This is the first study to reveal the antiproliferative effect of P. vulgaris flower extract. Further studies are now needed to identify the cytotoxic molecules in the extract and their mechanisms. (C) 2018 Xi'an Jiaotong University. Production and hosting by Elsevier B.V.