In this paper, it is aimed to compare the analytically and experimentally identified dynamic characteristics and obtain updated finite element model of Komurhan Highway bridge located on Elazig-Malatya highway. Analytical model of the bridge is constituted using SAP2000 software and dynamic characteristics are determined. The experimental measurements are carried out by Operational Modal Analysis under traffic loads and dynamic characteristics are obtained experimentally. Vibration data are gathered from both box girder and bridge deck. Measurement time, frequency span and effective mode number are determined by consider similar studies and literature. Peak Picking method in the frequency domain is used for the output-only modal identification. At the end of the study, analytical and experimental dynamic characteristic are compared with each other and finite element model of the bridge is updated by changing some uncertainties parameters such as material properties and boundary conditions.