We have reported, the synthesis, characterization, aggregation, and investigation of anti-microbial and anti-fungal activity properties of new octasubstituted metallophthalocyanine compounds containing dibutyl 4,4'-[(1,2-phenylene)bis(oxy)dibenzoate)]2,3-dicarbonitrile units. CoPc and MnPc were synthesized by the cyclotetramerization of dinitrile with anhydrous CoCl2 or Mn(OAc)(2). Eight bacteria and one fungus were used to test antimicrobial activities of the newly synthesized CoPc and MnPc compounds. MnPc has two times effective than the compound CoPc for against bacteria and fungus. MnPc showed antifungal activity against Candida albicans. It has been previously proved in the literature that the isoindole ring in the structure of the compounds play an important role in antimicrobial activity.