Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2(T)

Yanmis D., KARAOĞLU H., ÇOLAK D. N., Sal F., ÇANAKÇI S., BELDÜZ A. O.

TURKISH JOURNAL OF BIOLOGY, cilt.38, sa.5, ss.586-592, 2014 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 38 Sayı: 5
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3906/biy-1403-76
  • Dergi Adı: TURKISH JOURNAL OF BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.586-592
  • Anahtar Kelimeler: Xylose isomerase, Anoxybacillus, characterization, thermophilic, GLUCOSE-ISOMERASE, ESCHERICHIA-COLI, BIOCHEMICAL-CHARACTERIZATION, SACCHAROMYCES-CEREVISIAE, MOLECULAR-CLONING, D-XYLULOSE, GENE, PURIFICATION, EXPRESSION, XYLA
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2(T) has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography techniques to homogeneity, and the biochemical properties of the enzyme were characterized. The optimum temperature of the enzyme was 85 degrees C and maximum activity was observed at a pH of 6.5. Its Km and Vmax values were calculated as 25 +/- 2 mM and 0.12958 +/- 0.002 mu mol/min/mg protein, respectively. The effects of various metal ions on the xylose isomerase were examined. Divalent cations Co2+, Mg2+, and Mn2+ were essential for xylose isomerase activity; however, bivalent metal ions (Ca2+, Hg2+, Ni2+, Zn2+, Fe2+, and Cu2+) showed inhibitory effects. This is the first report of characterization of the xylose isomerase of Anoxybacillus spp. According to results obtained from this study, xylose isomerase is a promising candidate for industrial applications in production of xylulose and ribose.