Influence of lignin and its degradation products on enzymatic hydrolysis of xylan


Kaya F., Heitmann J., Joyce T.

JOURNAL OF BIOTECHNOLOGY, cilt.80, sa.3, ss.241-247, 2000 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 80 Konu: 3
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1016/s0168-1656(00)00265-0
  • Dergi Adı: JOURNAL OF BIOTECHNOLOGY
  • Sayfa Sayıları: ss.241-247

Özet

The influence of lignin, lignin model compounds, and black liquor from the kraft pulping process on the hydrolysis of xylan by xylanase was investigated. Addition of vanillic acid, acetovanillone, and protocatechuic acid increased the rate of hydrolysis of xylan by as much as 18-50% at low concentrations, but reached maxims at about 0.05% concentration. Addition of vanillin caused a 15% improvement in xylan hydrolysis, while addition of guaiacol more than doubled the hydrolysis rate. Increasing concentrations of either lignin or black liquor also increased the hydrolysis rate of xylan. Circular dichroism spectroscopy indicated a change in the structure of xylanase in the presence of black liquor. (C) Published by Elsevier Science B.V.