Akademik Veri Yönetim Sistemi
Journal of Microbiology and Infectious Diseases, cilt.13, sa.1, ss.38-46, 2023 (Hakemli Dergi)
Objectives: Salmonella Enteritidis is a pathogen that causes
gastroenteritis and septicemia in humans and animals, causing worldwide
health implications. This pathogen often causes fatal foodborne diseases
in humans and animals. In recent years, new treatment strategies for
bacterial infections have been studied. Bacteriophages might be used as
an alternative strategy for battling against bacterial diseases.
Methods: A novel SP-T1 phage was isolated, and its lytic activity
against Salmonella Enteritidis strains and other enteric bacterial
species was evaluated. In addition to its kinetic and genetic
characteristics, it is in vivo cytotoxicity and ability to inhibit the
development of biofilms were investigated.
Results: The gene sequence of the major capsid protein confirmed that
the SP-T1 phage belonged to the family Siphoviridae. There was no
evidence that phage had a lytic effect on bacteria other than the type
of Salmonella used in this investigation. The phage exhibited a short
latent period of 15 to 20 min and a burst size of 10 plaque forming unit
per infected cell. The phage was resistant to temperatures between 15
and 37 °C and pH levels between 5 and 11. The SP-T1 phage exhibited no
adverse effects on EPC cell culture and reduced biofilm formation.
Conclusions: Because of its short latent period, appropriate burst size,
ability to inhibit biofilm formation, and sensitivity to various
temperatures and pH levels, the phage found in this study would be an
excellent candidate for phage therapy. These findings are beneficial for
improving the microbiological safety and quality of food and creating
efficient bacteriophage-based regulation in the food chain.