Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS

Yazıcı Bektaş N., Ersoy E., Boğa M., Boran T., Çınar E., Özhan G., ...More

Turkish Journal Of Chemistry, vol.1, no.1, pp.0-1, 2021 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 1 Issue: 1
  • Publication Date: 2021
  • Doi Number: 10.3906/kim-2104-17
  • Journal Name: Turkish Journal Of Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core
  • Page Numbers: pp.0-1
  • Karadeniz Technical University Affiliated: Yes


The study aims to determine the secondary metabolites of Hypericum androsaemum L. 30 extracts by liquid chromatography-high resolution mass spectrometry (LC-HRMS), and 31 investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was 32 evaluated by MTT assay, and apoptosis induction abilities on human prostate 33 adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, 34 major secondary metabolites were found as hederagenin (762 ± 70.10 μg/g) in the leaves 35 dichloromethane (LD), herniarin (167 ± 1.50 μg/g) in fruit dichloromethane (FD), (-)- 36 epicatechin (6538 ± 235.36 μg/g) in the leaves methanol (LM), (-)-epigallocatechin 37 gallate (758 ± 20.46 μg/g) in the fruit methanol (FM), and caffeic acid (370 ± 8.88 μg/g) 38 in the fruit water (FW), and (3313 ± 79.51 μg/g) in the leaves water (LW) extracts. LM 39 exerted strong antioxidant activity in DPPH free (IC50 10.94 ± 0.08 μg/mL), and ABTS 40 cation radicals scavenging (IC50 9.09 ± 0.05 μg/mL) activities. FM exhibited cytotoxic 41 activity with IC50 values of 73.23 ± 3.06 µg/mL and 31.64 ± 2.75 µg/mL on PC-3 and 42 Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 ± 43 18.9 μg/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction 44 ability on different cells, FM extract showed apoptosis induction activity with 64.75% on 45 PC-3 cells at 50 μg/mL concentration. The study provides promising results about the 46 potential of Hypericum androsaemum on cancer prevention.