Towards ex situ conservation of globally rare Turkish endemic Tripleurospermum fissurale (Asteraceae)

Cuce M., İNCEER H., Imamoglu K. V., Ergin T., ÜÇLER A. Ö.

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, vol.58, no.6, pp.1002-1011, 2022 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 58 Issue: 6
  • Publication Date: 2022
  • Doi Number: 10.1007/s11627-022-10289-2
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Page Numbers: pp.1002-1011
  • Keywords: Tripleurospermum fissurale, Endangered species, Ex situ, Cytogenetic stability, Micropropagation, IN-VITRO PROPAGATION, MEDICINAL-PLANTS, CYTOTOXIC PROPERTIES, MICROPROPAGATION, DISCIFORME, L., ANTHEMIDEAE, RESOURCES, CULTURES
  • Karadeniz Technical University Affiliated: Yes


A rapid and efficient in vitro micropropagation system was developed to conserve Tripleurospermum fissurale (Sosn.) E.Hossain (Asteraceae), a rare endemic species for Turkey. Murashige and Skoog basal medium (MS) supplemented with 4.7 mu M KIN was found to be the most appropriate basal medium with a 60% germination rate. MS medium supplemented with 4.9 mu M 2iP and 0.5 mu M IBA gave the highest shoot length of 54.3 +/- 3.53 mm. Furthermore, 4.4 mu M 6-BA combined with 0.5 mu M IBA was superior for the highest shoot number with 3.4 +/- 0.49 after 4-wk culture. Cytogenetic analyses indicated that all propagated plants have the same DNA ploidy level (x) and chromosome number (2n = 18) compared with the mother plants. After 4 wk, the rooting percentage achieved 100% in all tested rooting media. MS medium supplemented with 2.7 mu M NAA favored the highest root number, root length, and secondary root number with 3.44 +/- 0.5, 170.1 +/- 4.91 mm, and 26.1 +/- 1.65, respectively. Rooted plantlets were initially acclimatized and then transferred in greenhouse conditions. This method could be evaluated for ex situ conservation of rare endemic and endangered plant species.