Alleviating Effect of Alpha-Pinene on Testicular Torsion and Detorsion Injury in Rats


Demir S., Kazaz İ. O., Sağnak Yılmaz Z., Türkmen Alemdar N., Ayazoğlu Demir E., Tepe R. S., ...More

JOURNAL OF UROLOGICAL SURGERY, vol.10, no.3, pp.253-258, 2023 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 3
  • Publication Date: 2023
  • Doi Number: 10.4274/jus.galenos.2023.2022.0080
  • Journal Name: JOURNAL OF UROLOGICAL SURGERY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.253-258
  • Karadeniz Technical University Affiliated: Yes

Abstract

Objective: It was aimed to evaluate whether alpha-pinene (AP) had a beneficial effect in the testicular ischemia/reperfusion injury (IRI) model for the first time. Materials and Methods: Testicular malondialdehyde, total oxidant status, and total antioxidant status levels were determined using spectrophotometric methods. The superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPx), myeloperoxidase (MPO), 78-kDa glucose-regulated protein (GRP78), activating transcription factor 6 (ATF6), and C/EBP homologous protein (CHOP) levels were determined using the commercial enzyme-linked immunosorbent assay kits. Hematoxylin-eosin staining method was used in the histopathological evaluation. Results: In the IRI group, testicular MDA, MPO, GRP78, ATF6, and CHOP levels were significantly increased, while GSH and GPx levels were decreased compared with the control group (p<0.01). AP application restored these levels significantly (p<0.05). Johnsen scores were also significantly lower in the IRI group compared the control group (p<0.001), and AP treatment increased these scores significantly (p<0.001). Conclusion: These results suggest that AP can protect the testicular tissue against IRI by inhibiting oxidative stress, inflammation, and endoplasmic reticulum stress with its antioxidant properties, thus providing a molecular basis for a novel medical treatment of testicular IRI. Future studies are needed on other mechanisms of the protective effect of AP on the pathogenesis of testicular torsion.