Genome-based reclassification of <i>Anoxybacillus salavatliensis</i> Cihan et al. 2011 as a later heterotypic synonym of <i>Anoxybacillus gonensis</i> Belduz et al. 2003

Inan Bektas K., Guler H. İ., Canakci S., Belduz A. O.

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, cilt.116, sa.5, ss.415-423, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 116 Sayı: 5
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s10482-023-01813-4
  • Dergi Adı: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Environment Index, Food Science & Technology Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.415-423
  • Anahtar Kelimeler: Anoxybacillus gonensis, Anoxybacillus salavatliensis, Genome-based reclassification, LIPID-COMPOSITION, SP NOV., SEQUENCES, DATABASE, TREES
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

In the present study, we aim to clarify the taxonomic positions of Anoxybacillus salavatliensis DSM 22626(T) and Anoxybacillus gonensis G2(T) by using whole genome phylogenetic analysis, biochemical and chemotaxonomic characteristics. The genome sequences of A. salavatliensis DSM 22626(T) was not available in any database, so it was sequenced in this study. In phylogenetic trees drawn using whole genome sequences and 16S rRNA gene sequences, A. salavatliensis DSM 22626(T) and A. gonensis G2(T) clade together and showed high sequence similarity (99.3%) based on 16S rRNA gene. The average amino acid identity, average nucleotide identity and digital DNA-DNA hybridization values between A. salavatliensis DSM 22626(T) and A. gonensis G2(T) were found to be greater than the threshold values for species demarcation. Further, the phylogenomic analysis based on the core genome of the strains under study confirmed that A. salavatliensis DSM 22626(T) and A. gonensis G2(T) formed a monophyletic clade. Most phenotypic and chemotaxonomic features between both strains were almost identical except for a few exceptions. The present results show that A. salavatliensis DSM 22626(T) is a later heterotypic synonym of A. gonensis G2(T).