Microwave Assisted Production of Gold Nanoparticles From Catechin Extract


Serdar G. , Gül Kılınç G., Sökmen M.

3rd International Congress on Analytical and Bioanalytical Chemistry (3rd ICABC 2021), Elazığ, Turkey, 22 - 26 March 2021, pp.55

  • Publication Type: Conference Paper / Summary Text
  • City: Elazığ
  • Country: Turkey
  • Page Numbers: pp.55

Abstract

In this study, the synthesis and characterization of Au nanoparticles from green tea leaves collected the Eastern Black Sea region (Of-TRABZON) were investigated. We isolated catechin extract using Supercritical Fluid Extraction (SFE) equipped with a modifier pump was used. 10 g of dried sample was shaken in 200 mL distilled water containing citric acid (0.1 M) for 90 min at room temperature and extracted in a laboratoary microwave device (Milestone, Start S Microwave, USA) at 4 minutes, 600 W and left cooling. The extracts obtained by applying microwave extraction were completely dried in the lyophilizer and transferred to the SFE system. Caffeine was separated by applying at 250 bar, 60 ° C and for 3 hours and then the catechin extract was separated by applying 0.5 mL / min ethanol, modified CO2-SFE extraction under the same conditions1 . The catechin mixture was dissolved in water (2% w/v) and used for AuNP production. Different portions of extract solution was added to HAuCI4 solution (0,5 mM-1 mM) and the mixture was exposed to a household microwave at 90 W for 1–30 min. The color of the mixture started in pale yellow and become purple-red and most of the nanoparticle resonance absorbance were between 500 and 600 nm range 2 . Absorption spectra were measured on a Shimadzu UVP-1240 spectrophotometer. All experimental work was repeated three times