In this paper, Crepis setosa subsp. topaliana (Asteraceae), which is known as endemic to Greece, is recorded as a new taxon for the flora of Turkey. It grows in scrub and in cultivated and grassy roadside places in the northwest and western parts of Turkey. An extended description of C. setosa subsp. topaliana is presented here, together with an identification key. The conservation status of this subspecies and its chromosome number are indicated.