Three seasonal comprehensive evaluation process of Digitalis trojana Ivan's phenolics

TURUMTAY H., Turumtay E. A., Selvi E. K., ŞAHİN H., Sandalli C., Yazici Z. A.

INDUSTRIAL CROPS AND PRODUCTS, vol.94, pp.160-166, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 94
  • Publication Date: 2016
  • Doi Number: 10.1016/j.indcrop.2016.08.039
  • Journal Name: INDUSTRIAL CROPS AND PRODUCTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.160-166
  • Keywords: Antioxidant, Cinnamic acids, Digitalis, Phenolic compounds, LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, LEAVES, IDENTIFICATION, L., CONSTITUENTS, VARIABILITY, GLYCOSIDES, PLANTS, ACIDS
  • Karadeniz Technical University Affiliated: Yes

Abstract

Digitalis trojana Ivan, a member of Digitalis genus, has highly bioactive properties due to, efficient phenolics. These compounds have shown to convert into each other in a limited time. Seasonal fluctuations of this plant leaves have been studied during the summer season using the high performance liquid chromatography-tandem mass spectrometry. Caffeic acid in June (2.48 mg/g extract), p-coumaric acid in August (6.69 mg/g extract) and trans-ferulic acid in July (4.85 mg/g extract) were the main compounds in the non-hydrolysed extracts. These compounds were heavily found in the hydrolysed extracts in August (14.63, 18.29 and 5.70 mg/g extracts, respectively). In addition, analysed flavonoids were found to be highest in July. Total phenolic contents were measured spectrophotometrically by using Folin-Ciocalteu's phenol reagent. The spectrophotometrically results were lowest (e.g. 43.50 mg GAE/g extract) in July while those of the hydrolysed extracts were highest (e.g. 167.19 mg GAE/g extract) in June. (C) 2016 Elsevier B.V. All rights reserved.