The Antimicrobial, Anti-Quorum Sensing, and Anti-Biofilm Activities of Ethanolic Propolis Extracts Used as Food Supplements

KOLAYLI S., Esertas U. Z. U., KARA Y.

BIOLOGY BULLETIN, cilt.49, sa.SUPPL 3, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: SUPPL 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1134/s1062359022150134
  • Dergi Adı: BIOLOGY BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Animal Behavior Abstracts, Artic & Antarctic Regions, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Propolis extracts are the natural bee product most widely used as food supplements. The high antioxidant and antimicrobial properties of propolis derive from its polyphenol compositions. This study investigated the relationship between the phenolic composition and antimicrobial activities of four different prepared ethanolic propolis extracts. The composition of the samples was tested in terms of total phenolic content and total flavonoid content, and their phenolic compositions were analyzed using high performance liquid chromatography-photometric diode array detection. Ferric reducing/antioxidant capacity (FRAP) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging tests were used as antioxidant assays. The antimicrobial effects of the extracts were tested using bacteriostatic, bactericide, anti-quorum sensing (QS), and anti-biofilm tests. Staphylococcus aureus, Chromobacterium violaceum, Mycobacterium smegmatis, Bacillus cereus, Candida albicans and Candida parapsilosis were used in the agar well diffusion assay. Anti-QS and anti-biofilm activities were tested against Chromobacterium violaceum ATCC31532, Chromobacterium violaceum ATCC 12472, and Pseudomonas aeruginosa PAO1. The phenolic compositions and the antioxidant properties of the extracts varied. All propolis specimens exhibited antimicrobial activity against the strains studied, although that activity varied according to the propolis extract. The findings showed that the high phenolic content of the propolis extracts resulted in high antioxidant and high antimicrobial activity.