Electromyography (EMG) signals are becoming increasingly important in many applications, including clinical/biomedical, prosthesis or rehabilitation devices, human machine interactions, and more. In this study, it is aimed to transmit the real-time wireless surface EMG signals taken from the muscles around the eyelids (orbicularis oculi) and to detect whether the activity is on the left or right eye at the receiver. The purpose of wireless transmission is to monitor the EMG activity with a portable unit. In addition, while the general application of surface EMG (sEMG) is to use one electrode group for each muscle tissue, but in this study, it is aimed to acquire signals separately from the muscles around the left and right eyelids by using a single electrode group. Left and right winking gestures are successfully detected from the single channel signal with the help of the implemented software and hardware.