Effects of Rosmarinic Acid Against Nephrotoxicity Induced by Cyclophosphamide in Rats

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Çetinavcı D., Yenilmez E., Bıyık A. F., Alver A., Sağlam N.

International Congress of Future Medical Pioneers 2021, 8 - 09 May 2021, pp.77

  • Publication Type: Conference Paper / Summary Text
  • Page Numbers: pp.77
  • Karadeniz Technical University Affiliated: Yes


In this study, the protective effect of rosmarinic acid (RA) against renal damage caused by 

cyclophosphamide (CP) was investigated. For this purpose, histopathological and biochemical 

evaluations were made. 

18 Spraque Dawley male rats were randomly divided into three groups: Sham, CP and CP+RA. 

Sham group was given physiologic serum (PS) by gavage for 8 days from the 1st day of the 

experiment and PS was administered intraperitoneally on the 8th day of the experiment. CP group 

was given PS by gavage from the 1st day to the 8th day and a single dose of 200 mg/kg CP was 

administered on the 8th day of the experiment intraperitoneally. The CP+RA group was given 100 

mg/kg RA by gavage for 8 days from first day and 200 mg/kg CP intraperitoneally on the 8th day. 

On the 9th day of the experiment, the rats were sacrificed and the blood of the rats was taken into the 

blood tubes. Half of each left kidney removed was taken into a 10% formalin solution for fixation 

and histological evaluations. Kidney tissue samples were stained using hematoxylin and eosin (H&E) 

and periodic acid-Schiff (PAS) staining methods for light microscopic evaluation. Tissue MDA, 

SOD, CAT assessments were made from the other half of the left kidney. BUN and creatinine in 

blood serum samples were measured. 

In histopathological evaluations, tubular atrophy, glomerular damage, vascular congestion, 

interstitial inflammation, epithelial vacuolization, desquamation in tubular epithelium, leukocyte 

infiltration and hyaline material accumulation was observed in the kidney sections of the CP group. 

These disorders were significantly reduced in the CP+RA group compared to the CP group. In PAS 

stained sections, histopathological findings such as widespread shedding, vacuolization were detected 

in the tubular epithelium and disruption of brush border and basal membrane were observed in CP 

group. These disorders decreased in CP+RA group compared to CP group. Increased BUN value in 

CP group decreased in CP+RA group; whereas decreased SOD value in CP group increased in 

CP+RA group. 

Our results show that CP causes histopathological damage in the rat kidney and that RA treatment 

relatively corrects these disorders caused by CP.

Keywords: Rosmarinic acid, Cyclophosphamide, Toxicity, Kidney, Oxidative stress.