International Congress of Future Medical Pioneers 2021, 8 - 09 May 2021, pp.77
In this study, the protective effect of rosmarinic acid (RA) against renal damage caused by
cyclophosphamide (CP) was investigated. For this purpose, histopathological and biochemical
evaluations were made.
18 Spraque Dawley male rats were randomly divided into three groups: Sham, CP and CP+RA.
Sham group was given physiologic serum (PS) by gavage for 8 days from the 1st day of the
experiment and PS was administered intraperitoneally on the 8th day of the experiment. CP group
was given PS by gavage from the 1st day to the 8th day and a single dose of 200 mg/kg CP was
administered on the 8th day of the experiment intraperitoneally. The CP+RA group was given 100
mg/kg RA by gavage for 8 days from first day and 200 mg/kg CP intraperitoneally on the 8th day.
On the 9th day of the experiment, the rats were sacrificed and the blood of the rats was taken into the
blood tubes. Half of each left kidney removed was taken into a 10% formalin solution for fixation
and histological evaluations. Kidney tissue samples were stained using hematoxylin and eosin (H&E)
and periodic acid-Schiff (PAS) staining methods for light microscopic evaluation. Tissue MDA,
SOD, CAT assessments were made from the other half of the left kidney. BUN and creatinine in
blood serum samples were measured.
In histopathological evaluations, tubular atrophy, glomerular damage, vascular congestion,
interstitial inflammation, epithelial vacuolization, desquamation in tubular epithelium, leukocyte
infiltration and hyaline material accumulation was observed in the kidney sections of the CP group.
These disorders were significantly reduced in the CP+RA group compared to the CP group. In PAS
stained sections, histopathological findings such as widespread shedding, vacuolization were detected
in the tubular epithelium and disruption of brush border and basal membrane were observed in CP
group. These disorders decreased in CP+RA group compared to CP group. Increased BUN value in
CP group decreased in CP+RA group; whereas decreased SOD value in CP group increased in
Our results show that CP causes histopathological damage in the rat kidney and that RA treatment
relatively corrects these disorders caused by CP.
Keywords: Rosmarinic acid, Cyclophosphamide, Toxicity, Kidney, Oxidative stress.