Akademik Veri Yönetim Sistemi
Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, cilt.27, sa.3, ss.520-524, 2024 (ESCI)
Aims: The use of antibiotics, which have been used in the treatment of infections, has been increasing. Therefore, the occurrence of antibioticresistant bacteria and resistance genes, both in infections and in the environment, is also increasing. In settlements near the water, wastewater is commonly collected in certain centers and discharged into nearby waters after treatment. As a result, the waters are polluted with pathogenic and resistant microorganisms, posing a great danger to human health. In this study, it was aimed at determining the antibiotic resistance profiles of Gram-negative bacteria isolated from wastewater samples taken from Trabzon City Center. Material and methods: Samples taken from wastewater were inoculated on tryptic soy agar and eosin methylene blue media to analyze the growing microorganisms. Thirty-six Gram-negative bacteria were included in the study. Resistance in bacteria was investigated by the disk diffusion method. Microorganisms with resistance were identified with the MALDI-TOF MS and BD Phoenix automated microbiology systems, and resistance profiles were obtained with the automated systems. The presence of bla SHV, bla TEM, bla OXA, bla CTX-M, integrase 1, integrase 2, and integron in resistant bacteria was investigated by the PCR method, and the transmission mechanisms of these genes were examined by transformation and conjugation experiments. In addition, the production of Extended Spectrum Beta Lactamase (ESBL) in isolates resistant to antibiotics was investigated by a double -disc synergy test. Results: As a result, in the current study, antibiotic resistance was found in 14 of 36 isolates. The presence of ESBL and bla oxa was shown to be isolated. It was determined that two isolates had plasmids. It was also shown that ampicillin resistance in one isolate was transformed with a conjugative plasmid, and kanamycin resistance was transformed with a non -conjugative plasmid. Conclusions: The detection of the presence of plasmids in some isolates carrying resistance suggests that antibiotic resistance may spread among bacteria in wastewater and may have adverse effects on living things.