Biochemical Content (Fatty Acids, Sterols, Lipophilic Vitamins, Total Protein, MDA, GSH, GSSG) of Noctiluca scintillans in the Southeastern Black Sea


Sen Ozdemir N., CAF F., FEYZİOĞLU A. M., Yildiz İ.

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES, vol.17, no.2, pp.301-311, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 17 Issue: 2
  • Publication Date: 2017
  • Doi Number: 10.4194/1303-2712-v17_2_09
  • Journal Name: TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.301-311
  • Keywords: PUFA, phytosterols, toxicity, lipid oxidation, RED TIDE PHENOMENA, LIPID-PEROXIDATION, ALPHA-TOCOPHEROL, COASTAL WATERS, SURFACE CHLOROPHYLL, POPULATION-DYNAMICS, PLANT STEROLS, FRESH-WATER, SAGAMI BAY, DINOFLAGELLATE
  • Karadeniz Technical University Affiliated: Yes

Abstract

Noctiluca scintillans reached the highest abundance (5105 ind/m(3)) in April; and April was an important period in the Black Sea ecosystem because of the seasonal thermocline. While the relationship between temperature and abundance was important (P<0.05, r(2)=-0.8), the relationship was not important between salinity and abundance (P<0.05, r(2)=-0.06); and Chlorophyll-a (Chl-a) and abundance (P<0.05, r(2)=-0.2). It was determined that total lipid was 0.5% in wet weight (WW). It was shown that the main Saturated Fatty Acids (SFA) were 16:0 (24%) and 18:0 (6%), main Monounsaturated Fatty Acids (MUFA) were 18:1 omega-9c (22%) and 16:1 omega-7 (4%), main Polyunsaturated Fatty Acids (PUFA) were 18:2 omega-6c (19%) and 20:4 omega-6c (8%). Phytosterols were the most important sterols in N. scintillans; and stigmasterol was 60 mu g/gr and beta-sterol was 8 mu g/gr as the most important ones in phytosterols. alpha-tocopherol was the highest amount (10.8 mu g/g) in lipophilic vitamins. It was found that Malondialdehyde (MDA) was 65 mu g/g, total protein was 10 mg/g, Glutathione (GSH) was 10565 mu g/g and GSSG) was 49 mu g/g. Consequently, PUFA and alpha-tocopherol were the most important biochemical parameters and alpha-tocopherol may reduce toxicity in blooms of N. scintillans because of inhibitory effect of alpha-tocopherol on lipid oxidation. Additionally, we can say that N. scintillans is a resistant dinoflagellate against oxidative stress.