Akademik Veri Yönetim Sistemi
7. Uluslararası Muğla Arıcılık ve Çam Balı Kongresi, Muğla, Türkiye, 15 - 19 Kasım 2022, ss.28
Silks are obtained from various sources such as spiders, silkworms, scorpions, bees and flies. Honeybee
silk is produced from salivary glands known as labial glands. Just before the honeybee hatch, the larvae cover the
waxed walls of their comb cells with silk. Although the primary building material for their nests is beeswax, the
honeycombs are constantly modified with the addition of silk and propolis. As the combs are covered with silk, the
mass ratio of silk becomes higher than wax. Hence, in order to obtain pure bee silk, it is necessary to separate the
silk from the wax which can only be achieved by chemical methods and/or heat treatment applications. Silk is used
in enzyme blocking, bone-like material production, drug release, tissue scaffolding, and wound dressing. In recent
years, they have become very popular materials especially in tissue engineering and it has been stated that they
have the potential to be used in industrial applications. In this study, it was aimed to produce a new value-added
product by investigating the skin rejuvenation properties of raw honey bee silk taken from honeycombs by
incorporating it into the cream formulation. Raw honey bee silks were separated from beeswax and powdered and
included in the cream formulation at 1%, 2% and 3% ratios. Skin rejuvenation properties were investigated by
measuring hyaluronidase enzyme inhibition in both bee silk and the creams produced. Stability tests, antioxidant
capacity tests and total mesophilic aerobic bacteria and yeast-mold count analyzes of the creams were performed.
Phase separation was not observed during storage while the pH values of bee silk added creams varied between
4.67 and 4.70. FRAP analysis results were found in the sample range of 2.026-1.598 µmol Trolox/g and total
mesophilic aerobic bacteria and yeast-mold count analysis results were determined as <100 cfu/g in all samples.
The results of the study showed that bee silk, which is less well known compared to other insect silks, has a
antihyaluronidase activity. In addition, with the use of bee silk in cream formulation for the first time, it is a first in
the development of apitherapeutic cosmetic products based on honey bee silk.
Keywords: Antihyaluronidase activity, Honey Bee silk, Cream, Cosmetics