Finite element model updating is very effective procedure to determine the uncertainty parameters in structural model and minimize the differences between experimentally and numerically identified dynamic characteristics. This procedure can be practiced with manual and automatic model updating procedures. The manual model updating involves manual changes of geometry and analyses parameters by trial and error, guided by engineering judgement. Besides, the automated updating is performed by constructing a series of loops based on optimization procedures. This paper addresses the ambient vibration based finite element model updating of long span reinforced concrete highway bridges using manual model updating procedure. Birecik Highway Bridge located on the 814km of Sanhurfa-Gaziantep state highway over Firat River in Turkey is selected as a case study. The structural carrier system of the bridge consists of two main parts: Arch and Beam Compartments. In this part of the paper, the arch compartment is investigated. Three dimensional finite element model of the arch compartment of the bridge is constructed using SAP2000 software to determine the dynamic characteristics, numerically. Operational Modal Analysis method is used to extract dynamic characteristics using Enhanced Frequency Domain Decomposition method. Numerically and experimentally identified dynamic characteristics are compared with each other and finite element model of the arch compartment of the bridge is updated manually by changing some uncertain parameters such as section properties, damages, boundary conditions and material properties to reduce the difference between the results. It is demonstrated that the ambient vibration measurements are enough to identify the most significant modes of long span highway bridges. Maximum differences between the natural frequencies are reduced averagely from %49.1 to %0.6 by model updating Also, a good harmony is found between mode shapes after finite element model updating.