This study aims to examine classroom teachers' Technological Pedagogical and Content Knowledge (TPACK) on the basis of different variables. A total of 322 classroom teachers in the city of Trabzon participated in the study. An adapted Turkish version of the TPACK scale was used as the data collection instrument. The obtained data was analyzed using SPSS 15.0 software. Independent t-test, ANOVA and Mann Whitney U-tests were used for statistical analysis. The results indicate that there are meaningful relationships and significant differences between variables of gender, having an Internet connection and the use of an ICT lab in the school, the use of educational software and the sub-factors of the TPACK scale. It is recommended that more practical CPD (Continuous Professional Development) opportunities for classroom teachers be provided in order to exploit the pedagogical benefits of ICT in primary schools.