Akademik Veri Yönetim Sistemi
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, cilt.99, sa.6, ss.690-694, 2017 (SCI-Expanded)
Experiments were designed to examine the in vitro effect of cobalt on sperm motility of the endangered trout species (Salmo coruhensis). Sperm samples were diluted in an immobilizing solution, and activated in a motility-activation solution that was supplemented with cobalt at concentrations of 1, 10, 100 and 1000 mg/L. The percentage of motile sperm and duration of motility were determined. Cobalt concentrations of 1-100 mg/L had a positive effect on the percentage of motile sperm and duration of motility compared to the control group, while a concentration of 1000 mg/L resulted in decreases in these parameters (p < 0.05). The percentages of motile sperm at cobalt concentrations of 0, 1, 10, 100 and 1000 mg/L were 83.33% +/- 0.25%, 88.33% +/- 0.34%, 89.00% +/- 0.57%, 90.00% +/- 0.87% and 42.50% +/- 0.45%, respectively; and the time durations over which the sperm remained motile were 72.00 +/- 0.63, 74.83 +/- 0.28, 77.40 +/- 0.47, 81.14 +/- 0.78, and 50.25 +/- 0.67 s, respectively. This study has shown that sperm motility and duration were significantly enhanced (p < 0.05) at cobalt concentrations of 1, 10 and 100 mg/L, relative to controls, and significantly decreased at 1000 mg/L.