Akademik Veri Yönetim Sistemi
Journal of Apitherapy and Nature, cilt.7, sa.2, ss.85-116, 2024 (Hakemli Dergi)
The molecules that consist of propolis are generally polyphenols, and they have many activities such as antiviral, antibacterial and antifungal activities. In this study, it is aimed to investigate the inhibiting capacity of the interaction between ACE-2 and Spike RBD by propolis samples belonging to three different cities (Trabzon, Kocaeli, Kırıkkkale). After determining the ropolis sample exhibiting the highest inhibition effect (Kocaeli-1 propolis), the phenolics within aqueous and ethanolic extracts of propolis sample were identified by RP-HPLC-UV and radical scavenging activities, antioxidant capacities, total flavonoids (TFC), phenolic contents (TPC) were determined. Then, individual assessments of the inhibition effects of each phenolic compound were conducted with Spike S1 (SARS-CoV-2): ACE-2 Inhibitor Screening Colorimetric Assay Kit and supported by in silico docking studies. The substances with the greatest inhibitory effect are; protocathecuic acid, caffeic acid, and p-coumaric acid with the inhibition of 62.29%, 58.34%, and 59.20%, respectively. The lowest IC50 value of the flavonoids was found to be 0.89 mM with caffeic acid. In silico, in vitro experiments, and MTT analyses conducted have demonstrated that caffeic acid and protocatechuic acid can be utilized as highly active compounds against COVID-19.