Akademik Veri Yönetim Sistemi
1st JOINT INTERNATIONAL APITHERAPY CONGRESS, İstanbul, Türkiye, 20 - 26 Mayıs 2021, ss.111-115
The bee pollen today has begun to recognize as an important product by consumers and food
industry as the result of investigations of its nutritional and therapeutic properties. However,
three main parts of pollen wall, pollen coat, exine, and intine form a quite resistant structure to
prevent the intracellular compounds from digestive degradation. Fermentation, which is
considered to be an effective method of breaking down the exine layer of bee pollen, facilitates
the release of compounds from the pollen matrix and contributes to an increase in the fraction
absorbed during digestion. The fermented product produced by bees in the honeycomb cells
with pollen, honey and effect of various enzymes in their digestive systems is called bee bread.
This product has prompted researchers to adapt the fermentation in the hive to lab conditions.
Research interest has been raised about spontaneous fermentation and/or fermentation with
added bacteria of pollen. In this study, the effect of autoclave sterilization (121 °C, 15 min) on
microbial decontamination and bioactive properties of bee pollen was investigated. TPCs were
found as 8.95 mg GAE/g DW for non-processed pollen, 29.32 mg GAE/g DW for autoclaved
pollen. TPCs were 19.67, 32.09, 11.55 mg GAE/g DW for pollen substrates with pollen:water
ratios 1:1, 1:2, and 3:1 after autoclave sterilization, respectively. The FRAP values have been
shown statistically significant increase for all samples after sterilization. Non-processed pollen,
autoclaved pollen, pollen substrates with pollen:water ratios 1:1, 1:2, and 3:1 after autoclave
sterilization showed free radical scavenging activity on DPPH with SC50 7.66, 3.32, 3.27, 3.12,
and 4.90 mg/mL DW, respectively. The number of LAB in non-processed pollen on MRS is
13.4x104 CFU/g. Total mesophilic aerobic count in PCA is found as 21.7 x104 CFU/g. Yeastmolds
in MEA is 6.1 x105 CFU/g. Growth of microorganisms after autoclave sterilization was
not detected.