AgNP Synthesis Using Supercritical CO₂ Extract of Lilium ciliatum Leaves


Serdar G., Koç Keşir M., Tunalı F., Terzioğlu S.

8th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2025), Ankara, Türkiye, 17 - 19 Aralık 2025, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Karadeniz Teknik Üniversitesi Adresli: Evet

Özet

Abstract

In this study, silver nanoparticles (AgNPs) were synthesized using Lilium ciliatum leaf extract obtained through supercritical CO₂ extraction (SFE-CO₂). In the green synthesis approach, plant materials utilize their biological extracts as reducing and capping agents, making the process highly economical because it significantly lowers the overall cost of synthesis (1). Leaves collected from Haçkalı Baba Plateau (Trabzon, Türkiye) was dried, mechanically fragmented, and subjected to SFE at 150 bar and 40 °C to obtain a phytochemically rich extract. Aqueous solutions of the extract at 2% (w/v) concentration were mixed in volumes of 0.1–6 mL with AgNO₃ solutions prepared in the range of 0.01–10 mM. The reaction mixtures were subjected to microwave irradiation at 90 W for 1–60 minutes, during which the appearance of a characteristic yellow color indicated the formation of silver nanoparticles. UV-Visible spectroscopy in the range of 300–800 nm was employed to confirm the synthesis of AgNPs by monitoring the surface plasmon resonance (SPR) bands characteristic of silver nanoparticles (2). This study demonstrates the potential of Lilium ciliatum leaf extract as a natural reducing and stabilizing agent in green nanoparticle synthesis and provides an effective microwave-assisted approach for the rapid and environmentally friendly production of AgNPs.

Keywords: Lilium ciliatum leaf, AgNPs,  SFE, MAE.

Funding:  This study was supported by Scientific and Technological Research Council of Türkiye (TUBITAK) under the Grant   Number Project No: 124Z589. The authors thank to TUBITAK for their supports.